Search for: All records

Abstract Stability, long lifetime, resilience against clogging, low noise, and low cost are five critical cornerstones of solid‐state nanopore technology. Here, a fabrication protocol is described wherein >1 million events are obtained from a single solid‐state nanopore with both DNA and protein at the highest available lowpass filter (LPF, 100 kHz) of the Axopatch 200B–the highest event count mentioned in literature. Moreover, a total of ≈8.1 million events are reported in this work encompassing the two analyte classes. With the 100 kHz LPF, the temporally attenuated population is negligible while with the more ubiquitous 10 kHz, ≈91% of the events are attenuated. With DNA experiments, the pores are operational for hours (typically >7 h) while the average pore growth is merely ≈0.16 ± 0.1 nm h⁻¹. The current noise is exceptionally stable with traces typically showing <10 pA h⁻¹increase in noise. Furthermore, a real‐time method to clean and revive pores clogged with analyte with the added benefit of minimal pore growth during cleaning (< 5% of the original diameter) is showcased. The enormity of the data collected herein presents a significant advancement to solid‐state pore performance and will be useful for future ventures such as machine learning where large amounts of pristine data are a prerequisite.